Cheyenne Mountain Zoo’s conservation team recently returned from Panama. The trip highlighted CMZoo’s commitment to support ongoing research, education and breeding efforts for amphibians facing threats in the wild.

As part of the Panama Amphibian Rescue and Conservation (PARC) project, Cheyenne Mountain Zoo is actively involved in saving 12 top-priority species of Panamanian amphibians threatened by an infectious fungus called chytrid.

Thanks to the breed and release work done with Wyoming toads, CMZoo and its partners have played an important role in helping support PARC. While the CMZoo conservation team was in Panama, they shared valuable knowledge and provided a case study to help further release strategies and future planning. With the sharing of information and financial support, we are excited to help develop future release plans for PARC.

Amphibians worldwide are facing a mass extinction, and by visiting CMZoo, you’re supporting teams focused on finding solutions. This trip was funded by our Quarters for Conservation program. Every time you visit the Zoo, you are making a difference for Panama frogs and many other species around the world.

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Every visit to the Zoo is conservation in action. Cheyenne Mountain Zoo and its guests and members are celebrating a huge milestone, having raised $3 million since the Zoo’s Quarters for Conservation program launched in 2008.

Quarters for Conservation, or Q4C, is the Zoo’s largest fundraiser for field conservation. It actively engages visitors and staff in supporting long-term projects championed by the Zoo. Known as legacy projects, these currently include biodiversity conservation on behalf of giraffe, Panama frogs, orangutans, black-footed ferrets, African elephants and rhinos, Wyoming toads and African vultures. Every visitor to the Zoo receives three “quarter” tokens representing the 75¢ Q4C allocation from their admission fee. They can then select the legacy projects they would like to support by placing their tokens in the corresponding slots in the Q4C kiosks. The kiosks record the number of tokens, so it’s easy to see how popular each project is.

“Our guests have helped us save animals from extinction simply by visiting the Zoo,” said Dr. Liza Dadone, vice president of mission and programs. “Three million dollars in conservation support is huge – and we want to thank our guests and members. Through this program, they are directly empowered to make a difference, and they did. It’s an example that no matter how small, when we all work together, we can change the world for the better.”

One of the projects that CMZoo recently supported and participated in on the front lines, is Operation Twiga. In November 2019, with financial support from Q4C, CMZoo staff traveled to Uganda for Operation Twiga IV. They helped transport 15 critically endangered Nubian giraffe to a safer home within Uganda, where their species has another chance at survival. As part of this project, CMZoo vet staff helped to collect important medical data for an ongoing study to help giraffe in their natural habitat and those in human care.

“We haven’t been involved in giraffe conservation that long, so this Q4c funding since 2008 has allowed us to really step up and take a leading role among Zoos in the past few years,” said Dr. Dadone. “It’s raised visibility in our Zoo community that giraffe populations aren’t safe. People see a large herd of giraffe here at the Zoo, and I think it’s easy to assume that they’re still doing okay across their native lands in Africa, but that’s no longer a reality. Giraffe are locally extinct in seven countries in Africa. Our Zoo, including our supportive community, is committed to ensuring that incredible animals like giraffe are around for the next generation.”

Q4C beneficiary species truly run the gamut, from 18-foot-tall giraffe in Africa, to tiny toads from Wyoming.
Staff member holding a Wyoming toad releasing them into the wild
“Another program I’m especially proud of is our Wyoming toad breed and release program,” said Dr. Dadone. “This species was once thought to be extinct in the wild. The only reason they exist in the wild today is because of our work and our team’s collaborations with other institutions. Some might think Wyoming toads aren’t as cute as a baby giraffe, but the toadlets are really adorable and are critical to our ecosystem and to ensuring we have a viable wildlife population in our own backyard. The research that we’ve done on headstarting [raising the toads to adulthood in the Zoo and then releasing them] and their nutrition has really improved the overall health of the last few generations of the Wyoming toad, which gives them an advantage when they are released into the wild. We’re continuing to evaluate our best practices and have an even better chance at saving this species long-term.”

Wyoming toads are symbolic of so many amphibian species in decline all over the world, including Panamanian frogs, which also receive support thanks to Q4C funds and frontline CMZoo staff support. In February 2019, three CMZoo staff members went to Panama to assist the Panama Amphibian Rescue and Conservation Project by remodifying two feeder insect pods into frog pods. For this 10-day trip, the goal was to set up two shipping containers to house and breed approximately 450 additional frogs brought in from El Valle Amphibian Conservation Center, and later released. This involved disinfecting the shipping containers, painting, installing plumbing and water filtration, assembling racks, and drilling and prepping tanks.
Black-footed ferret portrait
Q4C supports an in-house breed and release program for black-footed ferrets, as well. Since 1991, when CMZoo began breeding black-footed ferrets, 567 kits have been born. Roughly half of those kits are released into the wild while the others continue breeding at CMZoo and other facilities who support this recovery effort and the Species Survival Plan. This ongoing recovery effort supports the population of black-footed ferrets, who were once thought to be extinct, and which are vital to the prairie ecosystem in Wyoming.

CMZoo’s work to save habitats for orangutans through advocacy for sustainable palm oil production is largely supported by Q4C. Thanks to that financial support, CMZoo’s sustainable palm oil team consults staff at other conservation organizations on starting their own palm oil programs and recently attended the Roundtable on Sustainable Palm Oil’s (RSPO) annual conference in Thailand to represent CMZoo in the RSPO’s proceedings. Those proceedings directly impact the ways companies that use palm oil can help preserving wild lands for orangutans and other species.

Through Q4C, CMZoo also supports a conservation partner called Tsavo Trust – an organization in Kenya that works to protect the last of the big tuskers, which are African elephants with tusks weighing more than 100 pounds. CMZoo’s funds helped Tsavo Trust build permanent housing that allowed staff to live on the land where these critically endangered giants live. It also paid for pilot hours – frontline aerial surveillance that protects rhinos and elephants from poachers seeking their ivory.

The seventh Q4C legacy partner is VulPro – protectors of African vultures. The dedicated staff at VulPro, in South Africa, save vultures who have been injured as wild birds, and rehabilitates them to release. Those that are too injured to survive in the wild find a permanent home at VulPro.

Before launching Q4C in 2008, CMZoo was supporting conservation, but at a fraction of what is possible now. In the past few years, CMZoo has collected about half a million dollars per year through Q4C. Even with our three-month COVID-19 closure this year, the Zoo was able to hit this substantial $3 million milestone as expected.

“Small change pooled together makes a big difference,” said Dr. Dadone. “Thanks to our guests and members, we have been able to support this amazing frontline work. When we work together, we can make a positive difference for our world.”

In addition to contributions from admissions to every Zoo visit and special event ticket, $2.50 from each individual plus membership, and $5 from each family or higher-level membership, is allocated to Q4C. EdVenture and Animal department programs also contribute to the cause.

For more information about these projects and Quarters for Conservation, visit cmzoo.org/conservation.

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A nine-day trip to the jungles of Panama to help release hundreds of critically endangered frogs into the wild is just part of the job for Cheyenne Mountain Zoo veterinarian Dr. Eric Klaphake.

As part of the Panama Amphibian Rescue and Conservation project (PARC), the Zoo is actively involved in helping save dozens of species of Central American frogs and toads threatened by an infectious disease called chytrid fungus. Dr. Klaphake is part of the research team investigating the fungus’ impact while trying to preserve what remains of these species. There are currently 13 critically endangered frog species in human care at PARC, but the group is studying many more at risk.

The January release was composed of 500 Atelopus limosus frogs, also known as harlequin frogs or clown frogs due to their bright coloring. While not the first release of harlequin frogs by PARC, it was the largest. Like many release trials, it is uncertain how many may survive, as their resistance to chytrid is still in question. Also, determining perfect release sites for the frogs can be difficult. A site can appear good initially, but new issues can be revealed once it’s put into practice. However, it will provide the PARC researchers with valuable data.

“We will gain critical information for future releases, including understanding the challenges faced by a frog as it transitions from captivity into the wild,” said Dr. Klaphake.

Before being released, the frogs were injected with a harmless, under-the-skin fluorescent “tattoo” so they could be identified by black light upon recovery. In addition, a subgroup of about 30 frogs were outfitted with miniscule radio transmitters. The transmitters will provide valuable feedback, even though expense and limited battery life (approximately 21 days) limited the size of the subgroup.

“The transmitters will help the consortium understand where the frogs go after release, including how they respond to weather, what type of vegetation/location they like to use, and their preferred basking temperature,” explains Dr. Klaphake. “They will also make it easier to find those frogs to confirm life/death, and, if dead, hopefully discover whether chytrid, predation, or other causes contributed to death to help us better guide future releases.”

The frogs were released near the Cobre mine in Panama’s Colon province. Cobre is one of the largest copper reserves in Panama and the mining company contributes generously to PARC.

“In addition, it’s well secured, so it was a location we knew would not be easily disturbed by outsiders,” said Dr. Klaphake.

While January is considered the dry season, the weather was abnormally cool and rainy during Dr. Klaphake’s visit. Traversing the site involved wading up a creek, traversing its steep, clay banks, and battling the dense jungle understory. Along the way, the team observed other frog species, giant millipedes, rats, and baby anole lizards. They were careful to give wide berth to the few venomous fer-de-lance snakes they encountered.

As a founding partner for PARC, Cheyenne Mountain Zoo has provided instrumental support to the project, including participation in field expeditions and surveys, development of education programs, and veterinary and husbandry support in Panama. Dr. Klaphake has visited the PARC project in Panama once before, while Cheyenne Mountain Zoo Conservation Coordinator Jeff Baughman travelled there last year to participate in a smaller release.

The project is considered significant due to the immediate, and not inconsequential, threat of losing multiple amphibian species forever. Frogs are considered an indicator animal, meaning if they were to go extinct in Central America, eventually the delicate balance of our ecosystem would be upset and we would start to see a ripple effect caused by their absence.

While researchers in the PARC consortium and around the world are investigating how to halt the spread of chytrid and/or increase amphibians’ immunity to the fungus, there are other important steps we can take in our everyday lives to help amphibians thrive.

“Remember, all water flows downstream, so being careful about what we dump into sewers, drains, and waterways is important,” notes Dr. Klaphake, adding “especially medications – they can concentrate and lead to many problems, including fertility issues in many aquatic species, even at very low levels.”

To learn more about the threat to Panama frogs, visit http://amphibianrescue.org. Panama Frog Conservation is one of the Cheyenne Mountain Zoo’s Quarters for Conservation projects, so you can also choose to support our work with PARC every time you visit the Zoo.

Throughout Panama, a silent extinction is occurring. Dozens of frog and toad species have already been driven to extinction by chytridiomycosis, the world’s most lethal amphibian disease. Fortunately, amphibian conservationists and the greater zoo community have not been sitting idly by.

Caused by the chytrid fungus, this disease is responsible for having caused amphibian population declines in North, South and Central America, Australia, Europe, Africa and more. But in Panama, a ray of hope in the form of the Atelopus limosus has been released back out into the wild.

Jeff Baughman, Cheyenne Mountain Zoo’s Conservation Coordinator, was instrumental in the release, which was done in partnership with the Panama Amphibian and Rescue Conservation (PARC) Project, a program Cheyenne Mountain Zoo helped found.

Not only is CMZoo a founding partner for PARC, Baughman and other CMZoo staff have provided instrumental leadership to get the project at a point where the project can allow releases to happen. CMZoo staff assist with field expeditions and surveys, development of education programs and provide veterinary and husbandry support in Panama. This contribution has resulted in developing long-term security for Panama’s biodiversity.

PARC is truly an ark for amphibians. They partner with conservation organizations around the world to ensure the global survival of amphibians – focusing on those that can no longer be safeguarded in nature. In eastern Panama, there are 30 amphibian species that have been identified by PARC as rescue priorities.

This time, Baughman was on hand to release 90 of the Atelopus limosus, a native species more commonly known as the Limosa harlequin frog. A few years prior, only a handful of the species remained in existence.

“They’re very lucky and have a second chance,” Baughman explained. “It was nearly extinct, so we pulled the remaining few out of the wild to try and save the species.”

Baughman said conserving this particular species was difficult, as its numbers were so low. He said ideally conservationists aim to collect at least 20 males and 20 females of a species in order to generate greater genetic diversity in their offspring, yet there were so few of the Atelopus limosus collected during the first few expeditions and, their goal became simply to save it.

“We didn’t know how many other nearby sites would still have the limosus present. Time was ticking fast,” Baughman said.

The frogs were treated for the chytrid in an anti-fungal bath when they were first brought into the facility. Additionally, biosecurity is extremely important at the PARC facility. Scientists and workers disinfect any equipment or supplies that may have come into contact with the fungus, and remove shoes every time when entering the building. Baughman said humans could bring chytrid into the facility, so these precautions are necessary and crucial.

Baughman said the idea of releasing the species at lower elevations is interesting to the PARC scientists and others involved in the project because chytrid is less stable at areas that are warmer throughout the year. They released all of the frogs at the same location – within the lowland habitat, where temperatures are higher. They’re hopeful the released frogs will be able to self-treat if they happen to contract the disease again, as they’ll be in a warmer climate.

“The best case scenario we can hope for is that they survive and successfully reproduce,” Baughman said. “Even if this release trial isn’t successful this time, the information that is being collected will help not only this project, but many other amphibian programs throughout the world.”

The frogs will be tracked daily through July. There are a few frogs that have radio telemetry transmitter belts. They also have two forms of visible implant elastomer (VIE) markings for easier tracking. There are colored tags that are visible externally and are made up of a two-part elastomer material. One is an elastomer liquid that is injected into tissue with a hypodermic syringe and allows scientists to see the tags through a thin part of the frog’s skin. Within hours or days, the elastomer converts into a pliable solid and maintains the pigment in a well-defined mark, without damaging surrounding tissue or the animal.

“Think of it like a tiny fluorescent tattoo that can easily be seen under a black light,” he said.

Baughman traveled to Panama for the release itself, but also to help with the setup leading up to the release. Identifying and processing the animals took quite a bit of time, as did preparing and securing the actual release site.

There are currently 13 critically endangered frog species in human care at PARC. Baughman is hopeful for future releases for those species, as well.

Frogs are indicator animals, says Baughman. This means that if they were to go extinct in Central America, people would start to see a ripple effect caused by their absence.

“For example, you might start to see algae overgrowing in ponds, lakes and streams because there’d be no tadpoles to feed on it. That in turn would lead the PH and water chemistry to be off, so other species locally would start to suffer. Then unbalanced water would end up as runoff in the ocean and affect the species there.”

“It’s like a game of Jenga,” he explained. “You can pull one block here and there and the structure is still standing, but you’ll finally pull the one block that makes the whole thing collapse. Our ecosystem is such a delicate balance.”

To learn more about Panamanian frog conservation, please visit: http://amphibianrescue.org/